United States securities and exchange commission logo April 6, 2022 Randall C. Schatzman, Ph.D. Chief Executive Officer Bolt Biotherapeutics, Inc. 900 Chesapeake Drive Redwood City, California 94063 Re: Bolt Biotherapeutics, Inc. Registration Statement on Form S-3 Filed March 30, 2022 File No. 333-263994 Dear Dr. Schatzman: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Abby Adams at (202) 551-6902 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: John McKenna, Esq.